The 2025 Iowa State Cyclones football team represented Iowa State University as a member of the Big 12 Conference during the 2025 NCAA Division I FBS football season. The team was led by tenth-year head coach Matt Campbell, and played home games at Jack Trice Stadium in Ames, Iowa.
On December 5, Campbell was announced as the new head coach at Penn State. Days later, having finished their season with a winning record, the team opted out of a bowl appearance following a vote by players, citing a lack of healthy players to practice or play. On December 8, Washington State head coach Jimmy Rogers was announced as the Cyclones' new head coach.
The Iowa State Cyclones drew an average home attendance of 60,862, the 2nd-highest of all football teams from Iowa.