The 2025 season was the 10th season for FC Cincinnati and their 7th in Major League Soccer (MLS), the highest level of men's professional soccer in the United States. Their home stadium was TQL Stadium. It was the fourth season with the club for general manager Chris Albright and head coach Pat Noonan. Cincinnati commemorated their 10th season with a logo, along a series of patches that had represented different seasons in their first 10 years of history.
All matches are in Eastern Time
FC Cincinnati did not send their main squad the 2025 U.S. Open Cup as they were already participating in the 2025 CONCACAF Champions Cup and the 2025 Leagues Cup and sent FC Cincinnati 2 instead.
Numbers after plus-sign(+) denote appearances as a substitute.
|- ! colspan=16 style=background:#dcdcdc; text-align:center|Goalkeepers |-
|- ! colspan=16 style=background:#dcdcdc; text-align:center|Defenders
|- ! colspan=16 style=background:#dcdcdc; text-align:center|Midfielders
|- ! colspan=16 style=background:#dcdcdc; text-align:center|Forwards
|- |- ! colspan=16 style=background:#dcdcdc; text-align:center|Players who have played for FC Cincinnati this season but have left the club: