The 2025 Duquesne Dukes football team represented Duquesne University as a member of the Northeast Conference (NEC) during the 2025 NCAA Division I FCS football season. The Dukes were led by 21st-year head coach Jerry Schmitt and played their home games at Rooney Field in Pittsburgh, Pennsylvania.
The NEC released their preseason coaches' poll on August 4, 2025. The Dukes were picked to finish in second place.