The 2025 Columbus Crew season was the club's 30th season of existence and their 30th consecutive season in Major League Soccer, the top flight of soccer in the United States and Canada. The first match of the season was on February 22 against Chicago Fire FC. It was their third and final season under head coach Wilfried Nancy. The club revealed a logo to commemorate their 30th season.
Columbus Crew did not send their senior squad to the U.S. Open Cup as they were already participating in the 2025 CONCACAF Champions Cup and the 2025 Leagues Cup and sent Columbus Crew 2 instead.
Under "Apps" for each section, the first number represents the number of starts, and the second number represents appearances as a substitute.
|- |colspan=12 align=center|Players who left Columbus during the season:
Draft picks are not automatically signed to the team roster. Only those who are signed to a contract will be listed as transfers in. The picks for the Columbus Crew are listed below: