The 2025 Jonathan Fried Pro Challenger was a professional tennis tournament played on indoor hardcourts. It was the 16th edition of the tournament which was part of the 2025 ATP Challenger Tour, taking place in Charlottesville, United States from October 27 and November 2, 2025.
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following player received entry into the singles main draw through the College Accelerator programme:
The following player received entry into the singles main draw through the Next Gen Accelerator programme:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw: