The 2025 Cali Open WTA 125, also known as Kia Open, for sponsorship reasons, was a professional women's tennis tournament played on outdoor clay courts. It was the tenth edition of the tournament and part of the 2025 WTA 125 tournaments. It took place at the Club Campestre in Cali, Colombia between 27 October and 1 November 2025.
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser: