The 2025 I.T.C. Athens Challenger was a professional tennis tournament played on hardcourts. It was the first edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Athens, Greece between 24 and 30 November 2025.
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw through the Junior Accelerator programme:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw: