The 2024 Columbus Crew season was the club's 29th season of existence and their 29th consecutive season in Major League Soccer, the top flight of soccer in the United States and Canada. The first match of the season was on February 24 against Atlanta United FC. It was their second season under head coach Wilfried Nancy. This was the first season since 2016 without defender Josh Williams, who retired at the end of the previous season.
(on loan to Loudoun United) (on loan to Atletico Nacional) (on loan to Colorado Springs Switchbacks FC)
Columbus Crew 2 players Stanislav Lapkes and Owen Presthus joined the first team as guest players against Aston Villa.
Columbus Crew was not sent to the tournament, and neither was their MLS Next Pro team Columbus Crew 2 following the deal reached on March 1, 2024, due to participation in the 2024 CONCACAF Champions Cup.
Under "Apps" for each section, the first number represents the number of starts, and the second number represents appearances as a substitute.
|- |colspan=12 align=center|Players who left Columbus during the season:
Draft picks are not automatically signed to the team roster. Only those who are signed to a contract will be listed as transfers in. The picks for the Columbus Crew are listed below: