The 2023 Firenze Ladies Open was a professional tennis tournament played on outdoor clay courts. It was the first edition of the tournament and part of the 2023 WTA 125 tournaments, offering a total of $115,000 in prize money. It took place at the Match Ball Firenze Country Club in Florence, Italy between 15 and 21 May 2023.
The following players received a wildcard into the singles main draw:
The following players entered the main draw through protected ranking:
The following players received entry into the main draw through qualification:
The following player received entry as a lucky loser: