The 2021 Trofeo Alfredo Binda-Comune di Cittiglio was the 45th running of the Trofeo Alfredo Binda, a women's cycling race in Italy. It was the second event of the 2021 UCI Women's World Tour season and was held on 21 March 2021. The race started in Cocquio-Trevisago and finished in Cittiglio, on the outskirts of Lago Maggiore in Northwest Italy. The event was cancelled in 2020 due to the COVID-19 pandemic.
The race was won by Italian rider Elisa Longo Borghini of by over 90 seconds, after an attack on the penultimate lap on the Orino climb. It was Longo Borghini's second victory at Trofeo Binda, following her victory in the 2013 edition.
22 teams competed in the race.
UCI Women's WorldTeams
UCI Women's Continental Teams