The 2021 Per sempre Alfredo was the 1st edition of the Per sempre Alfredo road cycling one day race, which was held on 21 March 2021, starting in the city of Florence and finishing in Alfredo Martini's home town of Sesto Fiorentino.
The race was won in a sprint by Matteo Moschetti () ahead of Mikel Aristi () and Samuele Zambelli (Italy).
Two UCI WorldTeams, seven UCI ProTeams, ten UCI Continental teams, and the Italian national team made up the twenty teams that participated in the race. All teams entered seven riders each, for a total of 140 riders, of which 113 finished.
UCI WorldTeams
UCI ProTeams
UCI Continental Teams
National Teams