The 2021 Murray River Open was a tournament on the 2021 ATP Tour. It was played on outdoor hard courts in Melbourne, Australia. It was organised as a lead-up tournament to the 2021 Australian Open, and was held at the same venue, due to other tournaments in Australia being cancelled as a result from the COVID-19 pandemic. This tournament took place simultaneously with the 2021 Great Ocean Road Open and the 2021 ATP Cup.
<sub>*per team</sub>
The following players received wildcards into the main draw:
The following players have been accepted directly into the main draw using a protected or a special ranking:
The following player received entry as an alternate:
The following pairs received wildcards into the doubles main draw:
The following pair received entry using a protected ranking: