The ninth edition of Dwars door Vlaanderen's women's race was held on Wednesday 31 April 2021. The race started and finished in Waregem, and it was won by Annemiek van Vleuten () ahead of Katarzyna Niewiadoma () in a sprint of two. Alexis Ryan () won the bunch sprint for third place.
Eight UCI Women's WorldTeams and twenty UCI Women's Continental Teams competed in the race. Most teams started with 6 riders, making for a total of 164 riders of which there were 108 finishers within the time limit and two non-starters.
UCI Women's WorldTeams
UCI Women's Continental Teams