The 2021 Amstelveen Women's Open was a professional women's tennis tournament played on outdoor clay courts. It was the first edition of the tournament which was part of the 2021 ITF Women's World Tennis Tour. It took place in Amstelveen, Netherlands between 5 and 11 July 2021.
The following players received wildcards into the singles main draw:
The following player received entry using a junior exempt:
The following players received entry as special exempts:
The following players received entry from the qualifying draw: