The 2016 Wuhan Open (also known as the 2016 Dongfeng Motor Wuhan Open for sponsorship reasons) was a women's tennis tournament played on outdoor hard courts between September 25 and October 1, 2016. It was the 3rd edition of the Wuhan Open, and part of the WTA Premier 5 tournaments of the 2016 WTA Tour. The tournament was held at the Optics Valley International Tennis Center in Wuhan, China.
The following players received wild card<nowiki>s</nowiki> into the main singles draw:
The following players received entry from the singles qualifying draw:
The following pair received wildcards into the doubles main draw:
The following pair received entry as alternates: