The 2016 Royal London One-Day Cup tournament was a limited overs cricket competition that formed part of the 2016 domestic cricket season in England and Wales. Matches were contested over 50 overs per side and have List A status. All eighteen First-class counties competed in the tournament. Defending champions Gloucestershire went out in the group stage, as Warwickshire Bears beat Surrey in the final.
The competition featured two groups of nine teams, with the top four teams from each group progressing to the quarter-finals. The groups for the 2016 season were based on a geographical split, with a North and South group.
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