The 2016 KPIT MSLTA Challenger was a professional tennis tournament played on hard courts. It was the third edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place at Shree Shiv Chhatrapati Sports Complex in Pune, India from 24 to 30 October 2016.
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw: