The 2016 Challenger Banque Nationale de Saguenay was a professional tennis tournament played on indoor hard courts. It was the 11th edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $50,000 in prize money. It took place in Saguenay, Quebec, Canada between October 17 and October 23, 2016.
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry by junior exempt: