The 2016 season was Club de Regatas Vasco da Gama's 118th year in existence, the club's 101st season in existence of football, and the club's 3rd season playing in the Brasileirão Série B, the second tier of Brazilian football.
The initial report was released on 26 January. The teams involved in the tournament are required to register a squad of a maximum 31 players in total, including 3 goalkeepers and at maximum 5 players under 20 years old (born 1 January 1996 or after).
|- ! colspan=16 style=background:#dcdcdc; text-align:center|Goalkeepers
|- ! colspan=16 style=background:#dcdcdc; text-align:center|Defenders
|- ! colspan=16 style=background:#dcdcdc; text-align:center|Midfielders
|- ! colspan=16 style=background:#dcdcdc; text-align:center|Forwards
|- ! colspan=14 style=background:#dcdcdc; text-align:center| Players of youth squads who have made an appearance or had a squad number this season
|- ! colspan=16 style=background:#dcdcdc; text-align:center| Players who have made an appearance or had a squad number this season but have transferred or loaned out during the season