The 2016 Bol Open was a professional tennis tournament played on outdoor clay courts in Bol, Croatia. The tournament's first edition was held in late April 1991, and then again every year from 1996 to 2003. The tournament returned for 2016 as part of the WTA 125K series.
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry as a lucky loser:
The following pairs received a wildcard into the doubles main draw: