The 2010 Tallahassee Tennis Challenger was a professional tennis tournament played on outdoor hard courts. It was part of the 2010 ATP Challenger Tour. It took place in Tallahassee, United States between April 17 and April 24, 2010.
The following players received wildcards into the singles main draw:
The following players received Special Exempt into the singles main draw:
The following players received entry from the qualifying draw:
Brian Dabul def. Robby Ginepri, 4–6, 4–0, Ret.
Stephen Huss / Joseph Sirianni def. Robert Kendrick / Bobby Reynolds, 6–2, 6–4