The 2009 AFC Champions League qualifying play-off took place between 18 and 25 February 2009. The winners of the play-off round advanced to the group stage of the 2009 AFC Champions League, while the losers in each round entered the 2009 AFC Cup group stage.
<section begin=Preliminary round />
|- !colspan="3"|East Zone
<section end=Preliminary round />
<section begin=Play-off round />
|- !colspan="3"|East Zone
|- !colspan="3"|West Zone
<section end=Play-off round />