The 2000 Ukrainian Amateur Cup was the fifth annual season of Ukraine's football knockout competition for amateur football teams. The competition started on 2 July 2000 and concluded on 15 October 2000.
The cup holders FC Shakhta Ukraina Ukrainsk did not enter.
In bold are clubs that are active at the same season AAFU championship (parallel round-robin competition).
The following is the bracket that demonstrates the last four rounds of the Ukrainian Cup, including the final match. Numbers in parentheses next to the match score represent the results of a penalty shoot-out.