A by-election for the United Kingdom parliamentary constituency of Preston was held on 23 November 2000, following the death of incumbent Labour Party Member of Parliament (MP) Audrey Wise. Labour candidate Mark Hendrick won the by-election, holding the seat comfortably albeit with a reduced vote share.
It was held on the same day as by-elections in Glasgow Anniesland and West Bromwich West, both also won by Labour.
Incumbent Labour Party MP Audrey Wise died on 2 September 2000, triggering a by-election to fill the seat. She had been the constituency's MP since 1987, and had previously served as MP for Coventry South West from 1974 to 1979.