The 2000 Melbourne Cup was the 140th running of the Melbourne Cup, a prestigious Australian Thoroughbred horse race. The race, run over , was held on 6 November 2000 at Melbourne's Flemington Racecourse.
It was won by Brew, trained by Michael Moroney and ridden by Kerrin McEvoy.
This is a list of horses which ran in the 2000 Melbourne Cup.