The 2000 AF2 season was the first season of the af2. It was succeeded by 2001. The league champions were the Quad City Steamwheelers, who capped off a perfect season with a victory over the Tennessee Valley Vipers in ArenaCup I. This is the only AF2 season lacking division alignment.
ArenaCup I was the 2000 edition of the af2's championship game, in which the National Conference runners-up Tennessee Valley Vipers were defeated by the National Conference Champions Quad City Steamwheelers in Moline, Illinois by a score of 68 to 59.