2-hydroxyquinoline 8-monooxygenase () is an enzyme that catalyzes the chemical reaction
The four substrates of this enzyme are 2-hydroxyquinoline, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are 2,8-dihydroxyquinoline, oxidised NAD<sup>+</sup>, and water.
The enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is quinolin-2(1H)-one,NADH:oxygen oxidoreductase (8-oxygenating). This enzyme is also called 2-oxo-1,2-dihydroquinoline 8-monooxygenase. It is an ironâÂÂsulfur protein.
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes , , and .