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2-Hydroxy-1,4-benzoxazin-3-one monooxygenase

2-Hydroxy-1,4-benzoxazin-3-one monooxygenase (, BX5 (gene), CYP71C3 (gene)) is an enzyme with systematic name 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one,NAD(P)H:oxygen oxidoreductase (N-hydroxylating). This enzyme catalyses the following chemical reaction

2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NAD(P)H + H<sup>+</sup> + O<sub>2</sub> 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + NAD(P)+ + H<sub>2</sub>O

2-Hydroxy-1,4-benzoxazin-3-one monooxygenase is involved in the biosynthesis of protective and allelopathic benzoxazinoids in some plants.

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