2-deoxy-D-gluconate 3-dehydrogenase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are 2-deoxy-D-gluconic acid and oxidised nicotinamide adenine dinucleotide (NAD<sup>+</sup>). Its products are 3-dehydro-2-deoxy-D-gluconic acid, reduced NADH, and a proton.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD<sup>+</sup> or NADP<sup>+</sup> as acceptor. The systematic name of this enzyme class is 2-deoxy-D-gluconate:NAD<sup>+</sup> 3-oxidoreductase. This enzyme is also called 2-deoxygluconate dehydrogenase. This enzyme participates in pentose and glucuronate interconversions.
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .