2-chlorobenzoate 1,2-dioxygenase () is an enzyme from the bacterium Burkholderia cepacia that catalyzes the chemical reaction
The four substrates of this enzyme are 2-chlorobenzoic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen and a proton. Its products are catechol, carbon dioxide, oxidised NAD<sup>+</sup>, and a chloride ion.
This enzyme is an oxidoreductase that uses molecular oxygen as oxidant and incorporates both its atoms into the starting material. The systematic name of its class is 2-chlorobenzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, dechlorinating, decarboxylating). This enzyme is also called 2-halobenzoate 1,2-dioxygenase. It can oxidise other 2-halo substituted benzoic acids.