In enzymology, 2-alkyn-1-ol dehydrogenase () is an enzyme that catalyzes the chemical reaction below:
The two substrates of this enzyme are 1,4-butynediol and oxidised nicotinamide adenine dinucleotide (NAD<sup>+</sup>). Its products are 4-hydroxybut-2-ynal, reduced NADH, and a proton.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD<sup>+</sup> or NADP<sup>+</sup> as acceptor. The systematic name of this enzyme class is 2-butyne-1,4-diol:NAD<sup>+</sup> 1-oxidoreductase.