In enzymology, 2'-hydroxyisoflavone reductase () is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are 2'-hydroxyformononetin, reduced nicotinamide adenine dinucleotide phosphate (NADPH), and a proton. Its products are vestitone and NADP<sup>+</sup>.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is vestitone:NADP+ oxidoreductase. Other names in common use include NADPH:2'-hydroxyisoflavone oxidoreductase, isoflavone reductase, and 2',7-dihydroxy-4',5'-methylenedioxyisoflavone reductase.