2'-deoxymugineic-acid 2'-dioxygenase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are 2'-deoxymugineic acid and oxygen. Its product is mugineic acid (a derivative of azetidine-2-carboxylic acid).
The enzyme is an alpha-ketoglutarate-dependent hydroxylase with systematic name 2'-deoxymugineic acid,2-oxoglutarate:oxygen oxidoreductase (2-hydroxylating). It is also called IDS3. The active site of this non-heme iron protein generates a ferryl intermediate where Fe(IV)=O is the species that transfers its oxygen to the substrate.
This mechanism requires 2-oxoglutaric acid to activate the iron oxygen complex, and this gives succinic acid and carbon dioxide when the second atom of the molecular oxygen is removed.