This is a list of the 40 players who earned their 2000 PGA Tour card through Q School in 1999.
<nowiki>*</nowiki>PGA Tour rookie in 2000<br/> T = Tied <br> Green background indicates the player retained his PGA Tour card for 2001 (finished inside the top 125). <br> Yellow background indicates the player did not retain his PGA Tour card for 2001, but retained conditional status (finished between 126-150). <br> Red background indicates the player did not retain his PGA Tour card for 2001 (finished outside the top 150).