The 1958 United States Senate election in Florida was held on November 4, 1958.
After fending off a primary challenge from former senator Claude Pepper, the incumbent senator Spessard Holland was easily re-elected to a third term in office.
The incumbent senator Holland, a firm conservative, was challenged by former senator Claude Pepper, who had been unseated in 1950. Holland had played a role in recruiting George A. Smathers to run against the liberal Pepper in that election. The two served as colleagues in the Senate from 1947 to 1951. 26.4% of the voting age population participated in the Democratic primary.
Hyzer was unopposed for the Republican nomination.