16S rRNA (cytosine<sup>1407</sup>-C<sup>5</sup>)-methyltransferase (, RNA m5C methyltransferase YebU, RsmF, YebU) is an enzyme with systematic name S-adenosyl-L-methionine:16S rRNA (cytosine<sup>1407</sup>-C<sup>5</sup>)-methyltransferase. This enzyme catalyses the following chemical reaction
The enzyme specifically methylates cytosine<sup>1407</sup> at C<sup>5</sup> in 16S rRNA.