1-Hydroxycarotenoid 3,4-desaturase (, CrtD, hydroxyneurosporene desaturase, carotenoid 3,4-dehydrogenase, 1-hydroxy-carotenoid 3,4-dehydrogenase) is an enzyme with systematic name 1-hydroxy-1,2-dihydrolycopene:acceptor oxidoreductase. This enzyme catalyses the following chemical reaction
The enzymes from Rubrivivax gelatinosus and Rhodobacter sphaeroides acts primarily on acyclic carotenoids.