In enzymology, a (R)-6-hydroxynicotine oxidase () is an enzyme that catalyzes the chemical reaction
The 3 substrates of this enzyme are (R)-6-hydroxynicotine, HO, and O, whereas its two products are 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one and HO.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with oxygen as acceptor. The systematic name of this enzyme class is (R)-6-hydroxynicotine:oxygen oxidoreductase. Other names in common use include D-6-hydroxynicotine oxidase, and 6-hydroxy-D-nicotine oxidase. It employs one cofactor, FAD.
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes , , and .